Specific growth rate and not cell density controls the general stress response in Escherichia coli.

media and specific growth rate selection for high-cell-density cultivation of recombinant escherichia coli producing hGM-CSF in fed-batch process

Physiological and Morphological Changes of Recombinant E. coli During Over-Expression of Human Interferon-g in HCDC

The objective of this research was to investigate the influence of the over-expression of recombinant interferon-g during high cell density cultivation on cellular characteristics of recombinant E. coli. Batch and fed-batch culture techniques were employed to grow Escherichia coli BL21 for production of human gamma-interferon in pET expression system. Final cell densities in batch and fed-batch...

Maximizing Production of Human Interferon-γ in HCDC of Recombinant E. coli

Tuning recombinant protein expression is an approach which can be successfully employed for increasing the yield of recombinant protein production in high cell density cultures. On the other hand, most of the previous results reported the optimization induction conditions during batch and continuous culture of recombinant E. coli, and consequently fed-batch culture have received less attention....

Maximizing Production of Human Interferon-γ in HCDC of Recombinant E. coli

Tuning recombinant protein expression is an approach which can be successfully employed for increasing the yield of recombinant protein production in high cell density cultures. On the other hand, most of the previous results reported the optimization induction conditions during batch and continuous culture of recombinant E. coli, and consequently fed-batch culture have received less attention....

Efficient Process Development of Recombinant Human Granulocyte Colony-Stimulating Factor (rh-GCSF) Production in Escherichia coli

Background: The protein hormone granulocyte colony-stimulating factor (GCSF) stimulates the production of white blood cells and plays an important role in medical treatment of cancer patients. Methods: An efficient process was developed for heterologous expression of human GCSF in E. coli BL21 (DE3). The feeding rate was adjusted to achieve the maximum attainable specific growth rate under crit...